, González-Fernández Carmen, Esteban M. Angeles, Cuesta Alberto.

Nowadays, nanoplastics (NPs) are the most abundant nanomaterials on the market. This generates, in addition to the indirect production derived from the mechanical, physical or biological degradation of plastic debris, a direct contribution of NPs to the oceans. Determining the effects that the presence of NPs can have on the environment and the organisms that inhabit it is of vital importance since, due to their small size, they are capable of crossing cell membranes and, depending on their physical-chemical characteristics, be accumulated in organisms. The aim of the present work is to evaluate the potential effect of different functionalized NPs on the gilthead seabream Sparus aurata new cell line derived from the brain (SaB-1). The seabream is one of the most commercial fish species of the Mediterranean area and thus it is of great economic interest. SaB-1 cells were exposed to different 50 nm polystyrene (PS) NP types (PS-Plain, PS-COOH, PS-NH2). The LC50 (12 µg/mL) concentration was used to evaluate the impact of NPs for 24 hours of exposure. A battery of gene markers of oxidative stress (mta, sod, cat, gr and gst) and apoptosis (bcl2) was studied through qPCR to identify the effect of NP on cells. Preliminary results evidenced significant impacts of NPs exposure on cell viability, which differed depending on the NP charge, being PS-NH2 that promoting the highest cell mortality. Apoptotic processes were strongly activated by NPs. A down-regulation of bcl2 transcription was observed upon exposure to PS-NH2 and PS-Plain. Conversely, the transcription of mta was up-regulated by all NPs (PS-Plain¿PS-NH2¿PS-COOH) while gr was significantly increased by PS-COOH and decreased by PS-Plain. The present work evidence that the effect of NP is strongly influenced by particle's type and more research on fate and impact of nanoplastics at cellular level is needed.

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